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Forage Fermentation Profiles
By Dr. David P. Casper, P.A.S., Dairy Nutritionist

The measurement of the fermentation profile of forages is another diagnostic tool to evaluate the performance characteristics of forages when fed to dairy cattle. A fermentation profile consists of measuring the pH and volatile fatty acid (lactic, acetic, and butyric acids) concentrations of ensiled forages. Agri-King has extensively used these assays in the development of Silo King. However, these assays have also been used in special situations by the Nutrition Department to evaluate performance and herd health problems when fermented forages are fed. This is why fermentation profiles have been gaining in popularity with both Agri-King's Area Managers and the Dairy Industry.

What information can a forage fermentation profile provide? To help answer this question, the fermentation profiles of 1483 haylage and 1348 corn silage samples were summarized and the results are presented in Table 1 and Figures 1 through 4. Caution must be used in evaluating these averages because the data set contains both excellent and poorly fermented forage samples. Therefore, Table 1 also contains the recommended guidelines for an ideal fermentation profile. While the averages for haylage and corn silage given in Table 1 are close to the recommended guidelines, a large range in the values was observed. Forages with fermentation profiles outside of the recommended guidelines can lead to performance problems in dairy cows.

TABLE 1
No. samples 1483 1348
ITEM HAYLAGE CORN SILAGE RECOMMENDED
Moisture, % 59.9 66.7 65 - 70
pH 4.9 4.0 <4.5
Lactic Acid, % 4.5 4.5 >4.0
Acetic Acid, % 2.1 2.0 <2.0
Butyric Acid, % 0.3 0.04 0.0

The greatest factor responsible for forages having a fermentation profile not achieving the recommended guidelines was moisture. The rate of decline and extent of the final pH of an ensiled forage depends on moisture. Harvesting forages at recommended moisture levels is crucial to achieving optimal fermentation profiles. Harvest moisture is mainly controlled by farm management and environmental factors. Figures 1 and 2 show that the lowest pH occurred in haylage at 60-65% moisture and 67-70% moisture for corn silage.

Figure 1
Figure 2

Forages with lower pH's are better preserved and more stable during feed out than high pH forages. High pH forages are harvested either too wet or too dry, which results in less than optimum fermentation profiles. High pH forages are not stable and are prone to spoilage, heat upon feed out, have a short bunk life, poor palatability, and cause low dry matter intakes, milk production and milk components. The measurement of forage pH along with moisture can be used to evaluate whether a forage is stable or prone to spoilage.

Lactic acid is the most effective acid for reducing silage pH, thereby conserving high quality forage. The higher the lactic acid the better the forage quality. High quality forages are essential to support high milk production, while minimizing acidosis and health problems. Harvesting forages outside of the recommended moisture concentration results in lower concentrations of lactic acid, while the highest lactic acid concentrations (Figures 3 and 4) occurred when forage were harvested at the proper moisture concentrations. Ensiled forages with the highest lactic acid concentrations tend to be more nutrient dense, more palatable and sweet smelling, which results in greater nutrient intakes.

Figure 3

Harvesting forages with higher than recommended moisture contents (> 70%) can result in a deleterious or abnormal fermentation resulting in high acetic and butyric acid concentrations. High pH (>5.0) and butyric acid concentrations usually occur together and would indicate that a clostridial fermentation has occurred. These forages may have a strong foul smell, are unpalatable to dairy cattle, and cause low dry matter intakes. The growth of Clostridia causes excessive protein breakdown, which can result in an ammonia type smell. The ingestion of clostridial forages may cause health problems, including death of the animal. In addition, high butyric acid forages are known to cause ketosis in transition dairy cows.

Knowing the pH and lactic acid concentrations of forages can provide additional information about the quality of that forage for meeting the nutrient requirements of dairy cattle. This information, in conjunction with current nutrient measurements, will provide better information for formulating dairy rations. The value of these tests will be to identify those forages that have undergone abnormal fermentation (i.e. high pH's, acetic acid, and butyric acid with low or little lactic acid) that can not be identified by standard nutrient assays (i.e. poorly fermented, but acceptable nutrient profile). Forages with high pH's and low lactic acid concentrations may need to be fed in limited amounts or eliminated from the ration.

Analyzing forages for their fermentation profile has been gaining in popularity as a diagnostic tool in evaluating animal performance related to forage quality. Harvesting forages at the recommended moisture concentrations in conjunction with the use of Silo King will result in forages with the highest possible lactic acid and lowest acetic (<2%) and butyric acid (< .4%) concentrations that will maximize nutrient intake by the dairy cow.

In summary, the forage fermentation profiles can be used to evaluate the harvesting, storage, and feeding quality of forages on your dairy operation. Agri-King has been working on the development of methods to rapidly analyze pH and lactic acid concentrations in forages within 24 hours. Very shortly, pH will be reported on the sample analysis page for fermented forages. In the near future, lactic acid concentrations will also be reported. Be watching for these values on your sample test reports. If questions arise regarding the interpretation of these values, please contact your Agri-King Area Manager or the Nutrition Department.


Agri-King, Inc., Box 208, Fulton, IL 61252  -  800.435.9560  -  info@agriking.com